A Histologic Comparison of Light Emitting Diode Phototherapy-treated Hydroxyapatite-grafted Extraction Sockets.

A Histologic Comparison of Light Emitting Diode Phototherapy-treated Hydroxyapatite-grafted Extraction Sockets.

May 18, 2023
Phyllis Chiu

A histologic comparison of light emitting diode phototherapy-treated hydroxyapatite-grafted extraction sockets. Brawn P, Kwong-Hing A. Implant Dent 2007; 16(2):204-11

Background: The stimulating effect of red and near-infrared (NIR) laser phototherapy on bone regeneration and growth has been shown in a number of in vitro and animal studies. However, the effect of NIR phototherapy on the bone regeneration of hydroxyapatite (HA) -treated extraction sockets has not been previously demonstrated.

Materials and methods: An investigational Biolux extraoral light emitting diode phototherapy device was used daily for 21 days postextraction and socket grafting with HA (Osteograf LD300) unilaterally. Bone regeneration of the phototherapy-treated and nontreated side was compared in same-mouth extraction sockets.

Results: Histologic evaluations showed enhanced bone formation and faster particle resorption associated with the phototherapy-treated socket graft compared with the non-phototherapy-treated socket.

Conclusions: The accelerated bone healing in the phototherapy-treated HA socket graft may provide faster implant placement compared to non-phototherapy-treated socket grafts.

  1. Freitas, N. R. de ., Guerrini, L. B., Esper, L. A., Sbrana, M. C., Dalben, G. da S., Soares, S., & Almeida, A. L. P. F. de .. (2018). Evaluation of photobiomodulation therapy associated with guided bone regeneration in critical size defects. In vivo study. Journal of Applied Oral Science, 26, e20170244. https://doi.org/10.1590/1678-7757-2017-0244

The repair of bone defects raises the interest of investigators in several health specialties. Grafting techniques with bone substitutes and laser therapies have been investigated to replace autogenous bone and accelerate the bone healing process.

To evaluate the effect of photobiomodulation therapy (PBMT) associated with guided bone regeneration (GBR) in critical size defects.

Material and Methods

The study was conducted on 80 male rats (Rattus norvegicus albinus, Wistar) submitted to surgical creation of a critical size defect on the calvaria, divided into eight study groups: group C (control – only blood clot); group M (collagen membrane); group PBMT (photobiomodulation therapy); group AB (autogenous bone); group AB+PBMT; group AB+M; group PBMT+M; group AB+PBMT+M. The animals were killed 30 days postoperatively. After tissue processing, bone regeneration was evaluated by histomorphometric analysis and statistical analyses were performed (Tukey test, p<0.05).


All groups had greater area of newly formed bone compared to group C (9.96±4.49%). The group PBMT+M (achieved the greater quantity of new bone (64.09±7.62%), followed by groups PBMT (47.67±8.66%), M (47.43±15.73%), AB+PBMT (39.15±16.72%) and AB+PBMT+M (35.82±7.68%). After group C, the groups AB (25.10±16.59%) and AB+M (22.72±13.83%) had the smallest quantities of newly formed bone. The area of remaining particles did not have statistically significant difference between groups AB+M (14.93±8.92%) and AB+PBMT+M (14.76±6.58%).


The PBMT utilization may be effective for bone repair, when associated with bone regeneration techniques.

  1. Rosso, M.P.d.O.; Buchaim, D.V.; Pomini, K.T.; Coletta, B.B.D.; Reis, C.H.B.; Pilon, J.P.G.; Duarte Júnior, G.; Buchaim, R.L. Photobiomodulation Therapy (PBMT) Applied in Bone Reconstructive Surgery Using Bovine Bone Grafts: A Systematic Review. Materials 201912, 4051. https://doi.org/10.3390/ma12244051

The use of low-level laser therapy (LLLT) with biomodulatory effects on biological tissues, currently called photobiomodulation therapy (PBMT), assists in healing and reduces inflammation. The application of biomaterials has emerged in bone reconstructive surgery, especially the use of bovine bone due to its biocompatibility. Due to the many benefits related to the use of PBMT and bovine bones, the aim of this research was to review the literature to verify the relationship between PBMT and the application of bovine bone in bone reconstruction surgeries. We chose the PubMed/MEDLINE, Web of Science, and Scopus databases for the search by matching the keywords: “Bovine bone AND low-level laser therapy”, “Bovine bone AND photobiomodulation therapy”, “Xenograft AND low-level laser therapy”, and “Xenograft AND photobiomodulation therapy”. The initial search of the three databases retrieved 240 articles, 18 of which met all inclusion criteria. In the studies concerning animals (17 in total), there was evidence of PBMT assisting in biomaterial-related conduction, formation of new bone, bone healing, immunomarker expression, increasing collagen fibers, and local inflammation reduction. However, the results disagreed with regard to the resorption of biomaterial particles. The only human study showed that PBMT with bovine bone was effective for periodontal regeneration. It was concluded that PBMT assists the process in bone reconstruction when associated with bovine bone, despite divergences between applied protocols.

  1. Ediz Deniz, Ahmet Arslan, Nurettin Diker, Vakur Olgac & Ertugrul Kilic (2015) Evaluation of light-emitting diode photobiomodulation on bone healing of rat calvarial defects, Biotechnology & Biotechnological Equipment, 29:4, 758-765, DOI: 10.1080/13102818.2015.1036774

One of the major problems in modern dentistry is the recovery of bone defects. The aim of this prospective experimental study was to evaluate the effect of light-emitting diode (LED) photobiomodulation therapy on bone healing of rat calvarial defects. Twenty-eight male Sprague Dawley rats were used for the study. Critical size defects with 5 mm diameter were made with a trephine bur used in a low-speed handpiece under continuous sterile saline irrigation on each side of the sagittal suture. All critical size defects on the right side were filled with corticocancellous bone graft material and all the defects on the left side were left empty. The animals were randomly divided into two groups of 14 rats each. Group I received LED therapy and Group II did not receive any therapy. OsseoPulse® LED device (Biolux Research Ltd.) 618 nm wavelength and 20 mW/cm2 output power irradiation was started immediately after the surgery and was applied for 20 minutes at 24-h intervals for 7 and 14 days. In each group, seven rats were sacrificed on the 8th day and the remaining rats were sacrificed on the 15th day. Bone healing of the non-grafted side was statistically significant in Group I on both 8th day and 15th day; on the other hand, in the grafted side, enhanced bone healing was dominantly observed on the 15th day in Group I, compared to Group II, although the difference was not significant. Within the limits of this study, the findings suggested that LED therapy might have a favourable effect in the early phase of bone healing.

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