A Histologic Comparison of Light Emitting Diode Phototherapy-treated Hydroxyapatite-grafted Extraction Sockets.

A Histologic Comparison of Light Emitting Diode Phototherapy-treated Hydroxyapatite-grafted Extraction Sockets.

May 18, 2023
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Implant, Science

A histologic comparison of light emitting diode phototherapy-treated hydroxyapatite-grafted extraction sockets. Brawn P, Kwong-Hing A. Implant Dent 2007; 16(2):204-11

Background: The stimulating effect of red and near-infrared (NIR) laser phototherapy on bone regeneration and growth has been shown in a number of in vitro and animal studies. However, the effect of NIR phototherapy on the bone regeneration of hydroxyapatite (HA) -treated extraction sockets has not been previously demonstrated.

Materials and methods: An investigational Biolux extraoral light emitting diode phototherapy device was used daily for 21 days postextraction and socket grafting with HA (Osteograf LD300) unilaterally. Bone regeneration of the phototherapy-treated and nontreated side was compared in same-mouth extraction sockets.

Results: Histologic evaluations showed enhanced bone formation and faster particle resorption associated with the phototherapy-treated socket graft compared with the non-phototherapy-treated socket.

Conclusions: The accelerated bone healing in the phototherapy-treated HA socket graft may provide faster implant placement compared to non-phototherapy-treated socket grafts.

  1. Freitas, N. R. de ., Guerrini, L. B., Esper, L. A., Sbrana, M. C., Dalben, G. da S., Soares, S., & Almeida, A. L. P. F. de .. (2018). Evaluation of photobiomodulation therapy associated with guided bone regeneration in critical size defects. In vivo study. Journal of Applied Oral Science, 26, e20170244. https://doi.org/10.1590/1678-7757-2017-0244

The repair of bone defects raises the interest of investigators in several health specialties. Grafting techniques with bone substitutes and laser therapies have been investigated to replace autogenous bone and accelerate the bone healing process.

To evaluate the effect of photobiomodulation therapy (PBMT) associated with guided bone regeneration (GBR) in critical size defects.

Material and Methods

The study was conducted on 80 male rats (Rattus norvegicus albinus, Wistar) submitted to surgical creation of a critical size defect on the calvaria, divided into eight study groups: group C (control – only blood clot); group M (collagen membrane); group PBMT (photobiomodulation therapy); group AB (autogenous bone); group AB+PBMT; group AB+M; group PBMT+M; group AB+PBMT+M. The animals were killed 30 days postoperatively. After tissue processing, bone regeneration was evaluated by histomorphometric analysis and statistical analyses were performed (Tukey test, p<0.05).

Results

All groups had greater area of newly formed bone compared to group C (9.96±4.49%). The group PBMT+M (achieved the greater quantity of new bone (64.09±7.62%), followed by groups PBMT (47.67±8.66%), M (47.43±15.73%), AB+PBMT (39.15±16.72%) and AB+PBMT+M (35.82±7.68%). After group C, the groups AB (25.10±16.59%) and AB+M (22.72±13.83%) had the smallest quantities of newly formed bone. The area of remaining particles did not have statistically significant difference between groups AB+M (14.93±8.92%) and AB+PBMT+M (14.76±6.58%).

Conclusion

The PBMT utilization may be effective for bone repair, when associated with bone regeneration techniques.

  1. Rosso, M.P.d.O.; Buchaim, D.V.; Pomini, K.T.; Coletta, B.B.D.; Reis, C.H.B.; Pilon, J.P.G.; Duarte Júnior, G.; Buchaim, R.L. Photobiomodulation Therapy (PBMT) Applied in Bone Reconstructive Surgery Using Bovine Bone Grafts: A Systematic Review. Materials 2019, 12, 4051. https://doi.org/10.3390/ma12244051

The use of low-level laser therapy (LLLT) with biomodulatory effects on biological tissues, currently called photobiomodulation therapy (PBMT), assists in healing and reduces inflammation. The application of biomaterials has emerged in bone reconstructive surgery, especially the use of bovine bone due to its biocompatibility. Due to the many benefits related to the use of PBMT and bovine bones, the aim of this research was to review the literature to verify the relationship between PBMT and the application of bovine bone in bone reconstruction surgeries. We chose the PubMed/MEDLINE, Web of Science, and Scopus databases for the search by matching the keywords: “Bovine bone AND low-level laser therapy”, “Bovine bone AND photobiomodulation therapy”, “Xenograft AND low-level laser therapy”, and “Xenograft AND photobiomodulation therapy”. The initial search of the three databases retrieved 240 articles, 18 of which met all inclusion criteria. In the studies concerning animals (17 in total), there was evidence of PBMT assisting in biomaterial-related conduction, formation of new bone, bone healing, immunomarker expression, increasing collagen fibers, and local inflammation reduction. However, the results disagreed with regard to the resorption of biomaterial particles. The only human study showed that PBMT with bovine bone was effective for periodontal regeneration. It was concluded that PBMT assists the process in bone reconstruction when associated with bovine bone, despite divergences between applied protocols.

  1. Ediz Deniz, Ahmet Arslan, Nurettin Diker, Vakur Olgac & Ertugrul Kilic (2015) Evaluation of light-emitting diode photobiomodulation on bone healing of rat calvarial defects, Biotechnology & Biotechnological Equipment, 29:4, 758-765, DOI: 10.1080/13102818.2015.1036774

One of the major problems in modern dentistry is the recovery of bone defects. The aim of this prospective experimental study was to evaluate the effect of light-emitting diode (LED) photobiomodulation therapy on bone healing of rat calvarial defects. Twenty-eight male Sprague Dawley rats were used for the study. Critical size defects with 5 mm diameter were made with a trephine bur used in a low-speed handpiece under continuous sterile saline irrigation on each side of the sagittal suture. All critical size defects on the right side were filled with corticocancellous bone graft material and all the defects on the left side were left empty. The animals were randomly divided into two groups of 14 rats each. Group I received LED therapy and Group II did not receive any therapy. OsseoPulse® LED device (Biolux Research Ltd.) 618 nm wavelength and 20 mW/cm2 output power irradiation was started immediately after the surgery and was applied for 20 minutes at 24-h intervals for 7 and 14 days. In each group, seven rats were sacrificed on the 8th day and the remaining rats were sacrificed on the 15th day. Bone healing of the non-grafted side was statistically significant in Group I on both 8th day and 15th day; on the other hand, in the grafted side, enhanced bone healing was dominantly observed on the 15th day in Group I, compared to Group II, although the difference was not significant. Within the limits of this study, the findings suggested that LED therapy might have a favourable effect in the early phase of bone healing.

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Dr Michael R Hamblin

Science and Research Advisor

Michael R Hamblin Ph.D. was a Principal Investigator at the Wellman Center for Photomedicine at Massachusetts General Hospital, an Associate Professor of Dermatology at Harvard Medical School for 25 years, and is now a Distinguished Visiting Professor at the University of Johannesburg, South Africa and affiliated faculty at Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran.

He gained his PhD in organic chemistry from Nottingham Trent University in England. His research interests include photodynamic therapy, photobiomodulation, drug delivery, nanomedicine, and tissue engineering. His research program was supported by NIH, CDMRP, USAFOSR and CIMIT.

He has published over 750 peer-reviewed articles, over 150 conference proceedings, book chapters and International abstracts and holds 10 patents. He is Editor-in-Chief of “Photobiomodulation, Photomedicine and Laser Surgery”, Associate Editor for 10 journals, on the editorial board of a further 60 journals. He has an h-factor 148 and > 88,000 citations. He has authored/edited 36 textbooks on PDT, photomedicine, and nanomedicine including 13 SPIE proceedings.

Dr Hamblin was elected as a Fellow of SPIE in 2011, received the 1st Endre Mester Lifetime Achievement Award from NAALT in 2017, the Outstanding Career Award from the Dose Response Society, the 1st Ali Javan award for Photobiomodulation Basic Science Research from WALT in 2018, and the Lifetime Achievement Award from European Medical Laser Association in 2022.

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PBM WHITE

A WHITE SMILE IS NOTHING SHORT OF A CONFIDENT SMILE

  • PBM White embraces the use of light technology to whitening smiles. Our unique gel removes years of stains caused by coffee, tea, wine, or smoking.
  • PBM White combines a unique 2 wave lengths technology and a proprietary gel formulation to  allow a fast whitening process without the pain associated with comparable treatments.

A WHITE SMILE IS NOTHING SHORT OF A CONFIDENT SMILE

  • PBM White  Carbimide peroxide level is LOW but efficiency is HIGH. The proprietary formulation allows higher efficiency due to interaction with the light energy.
  • The LOW peroxide level prevents sensitivity and pain associated with common
    whitening treatments and reduces tooth damage risks.

High Grade Device

  • High quality silicon device
  • Easy to use
  • Hands-free device

Special Proprietary Process

  • Gentle whitening formula gel
  • Whiter teeth in 10 days
  • Only 15 minutes a day. Can be done more than once per day.

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Why Use PBM Vibe

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  • Reduced discomfort and sensitivity
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Why Use PBM Implant

  • Photobiomodulation stimulates mitochondrial activity which stimulates bone regeneration and blood flow.
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PBM Implant uses 680 nm near-infrared (NIR) light, which is similar to heat lamps, to deliver energy gently but directly to the bone and tissue around the implant body. Similar to plants and photosynthesis, the tissue can absorb this light energy and convert it to chemical energy, which stimulates the cells for faster bone and soft tissue regeneration and faster implant stability.

Flexible & comfortable mouthpiece

  • Standard Size mouthpiece with 46 LED’s
  • Decrease discomfort and pain

Flexible & comfortable mouthpiece

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  • Powered by a strong external battery

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Why Light Therapy

PBM Ortho uses 850 nm near-infrared (NIR) light, which is similar to heat lamps, to deliver energy gently but directly to the bone and tissue around the roots of the teeth. Similar to plants and photosynthesis, the tissue can absorb this light energy and convert it to chemical energy, which stimulates the cells for faster bone and soft tissue regeneration and faster tooth movement.

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Gentle Therapy

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PBM ORTHO

Why Light Therapy

PBM Ortho uses 850 nm near-infrared (NIR) light, which is similar to heat lamps, to deliver energy gently but directly to the bone and tissue around the roots of the teeth. Similar to plants and photosynthesis, the tissue can absorb this light energy and convert it to chemical energy, which stimulates the cells for faster bone and soft tissue regeneration and faster tooth movement.
  • PBM Ortho is a home device for patient designed to be used for aligners and fixed appliances treatment.
  • PBM Ortho is available in two sizes for molar treatment if necessary.
  • PBM Ortho use an 850nm wavelength for optimal effect.
  • Photobiomodulation effect stimulates mitochondrial activity. Increased activity triggers an anti-inflammatory natural response that reduces pain
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  • Product is guaranteed 2 years from the time of first usage.

Flexible & comfortable mouthpiece

  • Standard Size mouthpiece with 42 LED’s
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Faster Treatment treatment times

  • Change aligners every 3 days (down from 8.5 days)
  • Cut treatment time by 2.2 fold when used with braces

Gentle Therapy

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  • One 4-minute treatment per day per arch

Dr Alan Kwong Hing

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the Founder of PBM Healing, graduated with his Doctor of Dental Surgery degree (with distinction) from the University of Western Ontario. He received the University Gold Medal and completed a concurrent master’s degree in Pathology. Dr. Al has earned multiple other degrees and received numerous awards, including the pre–Doctoral IADR Hatton award for his research on MRIs. He is a serial entrepreneur, had a diverse career as a solo dentist, and owned large multi-dental groups in Canada, the UK, and the US. He has owned dental labs and specialized orthodontic labs, a dental model company, and a company that manufactured orthodontic wires and accessories.

He was a KOL for NobelBiocare for many years and worked with the team to develop the NobelBiocare Replace Implant. He ran a mini dental residency to train dentists and their teams in dental implant surgery and restorative procedures.

He enjoys lecturing worldwide, focusing on applying Photobiomodulation in medicine and dentistry. Most notable is the application of PBM principles in the medical and dental devices that he has developed to help alleviate pain and enhance healing.

He also lectures on personal and practice development in his role as a Coach. He is a published author, most notably for the “E Myth Dentist” with Michael Gerber. His current book on General principles of Photobiomodulation is in its final draft stages.

Samuel Faveur

CEO

He is the CEO of PBM Healing,  a  Founding Partner, Director, and Chief Executive Officer of Mandarin Capital Ltd, a Hong Kong-based asset Management and advisory Company specialising in start ups and growth stage technology companies.

 Samuel began his career in 1999 as a research analyst for ING Barings in London before moving to ING Barings’ proprietary and market trading convertible bond desk. He was transferred to the Tokyo office in 2001 and, in 2003, joined Mizuho Securities in Hong Kong as Head of Asset Management. In this role, Samuel was tasked with setting up an internal hedge fund platform and managing two funds specializing in convertible bonds and volatility investments and additionally designed and implemented trading and operations infrastructures.

By the time Samuel left Mizuho Securities in 2010, he had held the title of Head of Asset Management and was registered Responsible Officer: Type 9. In 2011 Samuel briefly joined MF Global as Head of Derivatives with proprietary and client facilitation mandates. Before setting up Mandarin Capital Ltd in 2012, Sam held the position of Managing Director and Portfolio Manager at Richmond Asset Management.

A Histologic Comparison of Light Emitting Diode Phototherapy-treated Hydroxyapatite-grafted Extraction Sockets.